Live-cell imaging of cellular proteins by a strain-promoted azide-alkyne cycloaddition

Live and let dye: Three coumarin-cyclooctyne conjugates have been used to label proteins tagged with azidohomoalanine in Rat-1 fibroblasts. All three fluorophores labeled intracellular proteins with fluorescence enhancements ranging from eight- to 20-fold. These conjugates are powerful tools for visualizing biomolecule dynamics in living cells.

Beatty, K., Fisk, J., Smart, B., Lu, Y., Szychowski, J., Hangauer, M., Baskin, J., Bertozzi, C., Tirrell, D.
ChemBioChem
Volume: 11
Pages: 2092 – 2095
Date: October, 2010
ICB Affiliated Authors: David A Tirrell