Vitronectin-based, bio-mimetic encapsulating hydrogel scaffolds support adipogenesis of adipose stem cells

The breakthrough of induced pluripotent stem cell (iPSC) technology has raised the possibility that patient-specific iPSCs may become a renewable source of autologous cells for cell therapy without the concern of immune rejection. However, the immunogenicity of autologous human iPSC (hiPSC)-derived cells is not well understood. Using a humanized mouse model (denoted Hu-mice) reconstituted with a functional human immune system, we demonstrate that most teratomas formed by autologous integration-free hiPSCs exhibit local infiltration of antigen-specific T cells and associated tissue necrosis, indicating immune rejection of certain hiPSC-derived cells. In this context, autologous hiPSC-derived smooth muscle cells (SMCs) appear to be highly immunogenic, while autologous hiPSC-derived retinal pigment epithelial (RPE) cells are immune tolerated even in non-ocular locations. This differential immunogenicity is due in part to abnormal expression of immunogenic antigens in hiPSC-derived SMCs, but not in hiPSC-derived RPEs. These findings support the feasibility of developing hiPSC-derived RPEs for treating macular degeneration.

Clevenger, T. N., Hinman, C. R., Ashley, R. K., Burke, D. J., Hawker, C. J., Messina, D., Van Epps, D. and Clegg, D. O.
Tissue Engineering
Volume: 17
Number: 3
Pages: 353-359
Date: August, 2015
ICB Affiliated Authors: Dennis Clegg, Craig J Hawker