Harnessing nature’s anaerobes for biotechnology and bioprocessing

Abstract

Industrial biotechnology has the potential to decrease our reliance on petroleum for fuel and bio-based chemical production and also enable valorization of waste streams. Anaerobic microorganisms thrive in resource-limited environments and offer an array of novel bioactivities in this regard that could revolutionize biomanufacturing. However, they have not been adopted for widespread industrial use owing to their strict growth requirements, limited number of available strains, difficulty in scale-up, and genetic intractability. This review provides an overview of current and future uses for anaerobes in biotechnology and bioprocessing in the postgenomic era. We focus on the recently characterized anaerobic fungi (Neocallimastigomy-cota) native to the digestive tract of large herbivores, which possess a trove of enzymes, pathways, transporters, and other biomolecules that can be harnessed for numerous biotechnological applications. Resolving current genetic intractability, scale-up, and cultivation challenges will unlock the potential of these lignocellulolytic fungi and other nonmodel microorganisms to accelerate bio-based production.

ICB Affiliated Authors

Authors
Igor A. Podolsky, Susanna Seppälä, Thomas S. Lankiewicz, Jennifer L. Brown, Candice L. Swift, and Michelle O'Malley
Date
Type
Peer-Reviewed Article
Journal
Annual Review of Chemical and Biomolecular Engineering
Volume
10
Number
1
Pages
105-128
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