Diels–Alder Photoclick Patterning of Extracellular Matrix for Spatially Controlled Cell Behaviors

Abstract

Strategies that mimic the spatial complexity of natural tissues can provide cellular scaffolds to probe fundamental questions in cell biology and offer new materials for regenerative medicine. Here, the authors demonstrate a light-guided patterning platform that uses natural engineered extracellular matrix (ECM) proteins as a substrate to program cellular behaviors. A photocaged diene which undergoes Diels–Alder-based click chemistry upon uncaging with 365 nm light is utilized. By interfacing with commercially available maleimide dienophiles, patterning of common ECM proteins (collagen, fibronectin Matrigel, laminin) with readily purchased functional small molecules and growth factors is achieved. Finally, the use of this platform to spatially control ERK activity and migration in mammalian cells is highlighted, demonstrating programmable cell behavior through patterned chemical modification of natural ECM.

ICB Affiliated Authors

Authors
Sophia J. Bailey, Erik Hopkins, Naomi J. Baxter, Isobel Whitehead, Javier Read de Alaniz, Maxwell Z. Wilson
Date
Type
Peer-Reviewed Article
Journal
Advanced Materials
Volume
35
Number
46
Pages
2303453
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